產品編號 | bs-40296G-IRDye800CW |
英文名稱 | AffiniPure Goat Anti-Mouse IgG H&L(min X Hu, Bov, Hrs, Rb, Sw Sr Prot) antibody |
中文名稱 | IRDye 800cw標記的親和純化山羊抗小鼠IgG (H+L)(最小交叉反應:Hu, Bov, Hrs, Rb, Sw Sr Prot) |
別 名 | IRDye? 800CW Goat anti-Mouse IgG H+L; 山羊抗小鼠IgG H&L (IRDye? 800CW)預吸附二抗; |
抗體來源 | Goat |
克隆類型 | Polyclonal |
交叉反應 | Mouse |
產品應用 | WB=1:10000-25000,IF=1:200-1200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 狀 | Liquid |
濃 度 | 1.0 mg/ml |
免 疫 原 | Native Mouse IgG |
亞 型 | IgG1 |
純化方法 | affinity purified by Protein G |
緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol |
保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
產品介紹 | |
產品圖片 |
Sample:
Lane 1: Rat Cerebrum tissue lysates
Lane 2: Rat Heart tissue lysates
Lane 3: Human A549 cell lysates
Lane 4: Human Jurkat cell lysates
Lane 5: Human Huvec cell lysates
Lane 6: Human Hela cell lysates
Lane 7: Human U2os cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti- GAPDH (bsm-33033M) at 1/50000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG (bs-40296G-IRDye8)at 1/20000 dilution
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |