| 產(chǎn)品編號 | bs-60296G-HRP |
| 英文名稱 | F(ab')? Fragment Goat Anti-Mouse IgG H&L antibody |
| 中文名稱 | HRP標記的F(ab)2片段羊抗小鼠IgG H&L |
| 別 名 | F(ab)2 Fragment Goat Anti-Mouse IgG H&L (HRP); F(ab)2 Fragment Goat Anti-Mouse IgG H&L/HRP; F(ab)2 Fragment Goat Anti-Mouse IgG(H+L); |
| 抗體來源 | Goat |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse |
| 產(chǎn)品應(yīng)用 | IHC-P=1:1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Liquid |
| 濃 度 | 1.0 mg/ml |
| 免 疫 原 | Native Mouse IgG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4°C，
An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37℃，DAB was used as the chromogen。
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4°C，
An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37℃，DAB was used as the chromogen。
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
