| 產(chǎn)品編號(hào) | bs-1132R |
| 英文名稱 | Somatostatin 14/Somatostatin 28 Rabbit pAb |
| 中文名稱 | 生長(zhǎng)抑素抗體 |
| 別 名 | GRIH; Growth hormone release inhibiting factor; SMS_HUMAN; SMST; SST; Somatostatin 14; Somatostatin14; Somatostatin-14; somatostatin SS14; Somatostatin 28; Somatostatin28; Somatostatin-28; somatostatin SS28. |
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Specific References (2) | bs-1132R has been referenced in 2 publications.
[IF=4.26] Okita et al. Modified Western blotting for insulin and other diabetes-associated peptide hormones. (2017) Sci.Re. 7:6949 WB ; Mouse.
[IF=0.392] Yildiz, M.. et al. The Effect of Chronic Intermittent Cold Exposure on Gastrin-, Somatostatin-, Secretin-, and Serotonin-Containing Cells in the Small Intestine of Rats. Biol Bull+. 2021 Dec;48(3):S95-S106 IHC ; Rat.
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| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 生長(zhǎng)因子和激素 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Chicken) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:200-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Somatostatin 28: 103-116/116 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. [provided by RefSeq, Jul 2008].
Function: Somatostatin inhibits the release of somatotropin. Subcellular Location: Secreted. Similarity: Belongs to the somatostatin family. SWISS: P61278 Gene ID: 6750 Database links: Entrez Gene: 396279 Chicken Entrez Gene: 6750 Human Entrez Gene: 20604 Mouse Omim: 182450 Human SwissProt: P33094 Chicken SwissProt: P61278 Human SwissProt: P60041 Mouse Unigene: 12409 Human Unigene: 34418 Rat 生長(zhǎng)抑素是胰島D細(xì)胞的一種多肽激素，主要用于胰島細(xì)胞的功能性分類和消化道粘膜中內(nèi)分泌細(xì)胞及腫瘤的研究。 生長(zhǎng)抑素在體內(nèi)分布廣泛。在神經(jīng)系統(tǒng)中，廣泛存在于中樞和外周神經(jīng)系統(tǒng)，在腦內(nèi)以下丘腦正中隆起的濃度為最高。在新皮層，邊緣系統(tǒng)下杏仁核，海馬等部位也廣泛存在，以皮層含量最高。在脊髓后根和三叉神經(jīng)神經(jīng)節(jié)內(nèi)的一級(jí)神經(jīng)元中，亦含生長(zhǎng)抑素的免疫反應(yīng)性物質(zhì)。生長(zhǎng)抑素廣泛存在于胃腸道粘膜的“D”細(xì)胞，以胃竇和胃體最高，在腸內(nèi)越往下含量越低?！癉”細(xì)胞有長(zhǎng)的胞漿突起，在幽門腺區(qū)止于G細(xì)胞和“嗜鉻細(xì)胞”，在泌酸腺區(qū)止于壁細(xì)胞和其他上皮細(xì)胞。生長(zhǎng)抑素通過(guò)旁分泌機(jī)制由突起釋放到G細(xì)胞和壁細(xì)胞膜上，抑制胃泌素和HCI分泌，在胰腺內(nèi)，生長(zhǎng)抑素由胰島“D”細(xì)胞分泌，通過(guò)血液循環(huán)對(duì)胰島及消化道起作用，作為旁分泌調(diào)節(jié)胰島功能。 生長(zhǎng)抑素的生理作用為：抑制垂體生長(zhǎng)激素、促甲狀腺激素、促腎上腺皮質(zhì)激素和催乳類的釋放，也抑制各種胃腸激素的釋放，抑制胃泌素、促胰液素、膽囊收縮素、胃動(dòng)素、胰多肽、胰高血糖素、腸高血糖素等的釋放。同時(shí)也抑制胃酸、胃蛋白酶、胰蛋白酶及唾液淀粉酶的分泌。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Somatostatin/GRIH Polyclonal Antibody, Unconjugated(bs-1132R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Somatostatin 28) Polyclonal Antibody, Unconjugated (bs-1132R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
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